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. 2002 May;9(3):716–719. doi: 10.1128/CDLI.9.3.716-719.2002

FIG. 1.

FIG. 1.

Detection of BV and other alphaherpesviruses by PCR. (A) PCR with primers gGS4 (forward, 5′-CCGCGTACGACTACGAGATCC-3′) and gGAS4 (reverse, 5′-GTTCGCGGCCACGATCCA-3′) in the presence of 1.5 M betaine. After an initial denaturation step (94°C, 5 min), PCR was conducted for 35 cycles (94°C, 1 min; 55°C, 1 min; 72°C, 2 min), followed by a final extension (72°C, 7 min). PCR products were subjected to electrophoresis on a 5% polyacrylamide gel, and DNA bands were visualized under illumination at 254 nm after being stained with SYBR Green I (9). The arrow at the right indicates the position of the 209-bp fragment in lanes 1 and 3. (B) PCR with primers BV1 and BV2 tested according to the method of Scinicariello et al. (16, 17). The arrow indicates the 128-bp fragment. Lane M, HaeIII-digested φX174 replicative form (RF) DNA. Lanes 1 to 4, BV DNA as the PCR template. Lane 1, strain E2490 (rhesus macaque); lane 2, strain E90-136 (cynomolgus macaque); lane 3, strain 8100812 (lion-tailed macaque); lane 4, strain Kumquat (pigtail macaque); lanes 5 to 8, DNA of primate alphaherpesviruses other than BV as the PCR template; lane 5, human HSV-1 (strain KOS); lane 6, human HSV-2 (strain 186); lane 7, SA8 (strain B264) of green monkeys; lane 8, HVP2 (strain OU1-76) of baboons.