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PCR assays with the purified BHV2 DNA as the template. Lanes: M, HaeIII-digested φX174 RF DNA; 1, PCR with primers S4 and AS4 in the presence of 1.5 M betaine (9); 2, PCR with primers BV1 and BV2 tested according to the method of Scinicariello et al. (16, 17); 3, PCR with primers B3 and B4 tested according to the method of Black and Eberle (1). The arrow indicates the predicted 542-bp fragment in lane 3.
