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. 2025 Mar 10;301(4):108398. doi: 10.1016/j.jbc.2025.108398

Figure 4.

Figure 4

Effect of PDK1 suppression on triacylglycerol metabolism. Cells were incubated in 375 μM of palmitic acid conjugated to BSA (6:1 M ratio) in complete culture medium for 18 h. A and B, fatty acid oxidation dependency and (C and D) capacity were measured from oxygen consumption rate measurements of 6 independent co-cultures spanning 3 experimental repeats. E, Triacylglycerol abundance was measured following BSA or palmitic acid incubation in complete culture medium, (F) nutrient deprived in 5.5 mM glucose, serum free culture media for 3 h. G, thin layer chromatograph of alkyne incorporated lipids in cells treated with 10 μM of alkyne palmitate for 18 h, then nutrient deprived in low glucose, serum-free media for 3 h (n = 3). Data are shown as mean ± SEM and were analyzed by a Two-way ANOVA followed by a post hoc Tukey multiple comparison test. For (B), ANOVA showed (F and p values) of (0.066 and 0.7995), (0.002534 and 0.9604), (18.12 and 0.0004) for interaction, row (PA treatment) and column (cell lines) factors respectively. For (D), ANOVA showed (F and p values) of (1.326 and 0.2631), (40.91 and < 0.0001), (13.16 and 0.0017) for interaction, row (PA treatment) and column (cell lines) factors respectively. For (E), ANOVA showed (F and p values) of (0.1336 and 0.7190), (30.66 and < 0.0001), (0.4757 and 0.4992) for interaction, row (PA treatment) and column (cell lines) factors respectively. For (F), ANOVA showed (F and p values) of (30.91 and 0.0001), (250.2 and < 0.0001), (27.78 and 0.0002) for interaction, row (nutrient availability) and column (cell lines) factors respectively. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.