Figure 7.

Predicted enzymatic activity. Algorithmic prediction of enzyme activity based on our lipidomics dataset was performed on the LipidOne platform (v 2.1). Activity prediction was carried out in Pdk1 knockdown cells relative to control in the (A) palmitic acid-treated condition and the (B) nutrient-deprived condition. The color of the nodes represents the activation status in Pdk1 knockdown cells relative to the control. Nodes in cream represent proteins not identified by the algorithm but shown nonetheless to provide increased biochemical context. The color of the line around the node represents the fold change of the protein abundance in Pdk1 knockdown cells relative to the control derived from our proteomics dataset. The thickness of the line around the node represents the adjusted p-value.