TABLE 1.
ELISA anticapsular antibody concentrations in CDC1992 and 12 CDC quality control sera for meningococcal groups A, C, Y, and W-135
| Serum sample no. | Ab concentration (μg/ml) for meningococcal capsular polysaccharide [mean (SE)]a
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group Ab
|
Group Cb
|
Group Y
|
Group W-135
|
|||||||||||||
| IgG | IgM | IgA | Totalc | IgG | IgM | IgA | Totalc | IgG | IgM | IgA | Totalc | IgG | IgM | IgA | Totalc | |
| CDC1992 | 91.8 (8.7) | 23.9 (1.4) | 20.1 (1.2) | 135.8 | 24.1 (1.1) | 2.0 (0.1) | 5.9 (0.2) | 32.0 | 31.8 (2.4) | 2.9 (0.2) | 3.7 (0.4) | 38.4 | 16.2 (1.0) | 1.4 (0.1) | 2.5 (0.2) | 20.1 |
| 900215 | 515.5 (24.6) | 60.6 (0.8) | 34.3 (0.9) | 610.4 | 11.9 (0.4) | 16.7 (1.4) | 7.7 (0.6) | 36.3 | 35.1 (3.7) | 2.1 (0.2) | 9.7 (0.5) | 46.9 | 25.9 (2.6) | 3.2 (0.2) | 10.0 (1.3) | 39.1 |
| 900242 | 17.2 (0.6) | 35.0 (0.8) | 50.2 (1.4) | 102.4 | 119.9 (12.1) | 24.5 (1.1) | 101.9 (8.5) | 246.3 | 48.3 (2.7) | 2.2 (0.3) | 15.0 (0.9) | 65.5 | 4.5 (0.4) | 5.0 (0.5) | 4.7 (0.5) | 14.2 |
| 900243 | 24.5 (0.2) | 77.3 (1.4) | 9.8 (0.2) | 111.6 | 32.8 (1.8) | 9.5 (1.1) | 9.6 (0.6) | 51.9 | 134.1 (9.3) | 5.2 (0.6) | 6.2 (0.5) | 145.5 | 6.7 (1.1) | 4.1 (0.3) | 2.7 (0.2) | 13.5 |
| 900268 | 39.6 (2.0) | 18.6 (0.5) | 15.2 (0.3) | 73.4 | 38.0 (0.9) | 2.3 (0.2) | 21.0 (1.8) | 61.3 | 21.9 (2.1) | 2.8 (0.3) | 5.4 (0.2) | 30.1 | 8.7 (0.7) | 2.3 (0.1) | 1.9 (0.2) | 12.9 |
| 900274 | 143.6 (9.4) | 24.4 (2.7) | 7.4 (0.7) | 175.4 | 15.4 (1.3) | 11.1 (1.0) | 37.9 (4.1) | 64.4 | 4.2 (0.7) | 1.9 (0.2) | 2.3 (0.2) | 8.4 | 1.1 (0.1) | 1.3 (0.1) | 0.7 (0.1) | 3.1 |
| 900305 | 20.4 (0.8) | 25.7 (1.6) | 4.5 (0.5) | 50.5 | 66.6 (0.2) | 6.7 (0.6) | 14.4 (1.0) | 87.7 | 8.4 (1.3) | 10.7 (1.4) | 4.6 (0.3) | 23.7 | 2.2 (0.2) | 13.9 (0.7) | 1.2 (0.1) | 17.3 |
| 900306 | 1.5 (0.1) | 1.5 (0.1) | 0.6 (0.1) | 3.6 | 74.1 (6.2) | 0.3 (0.1) | 0.7 (0.1) | 75.1 | 279.2 (22.4) | 0.3 (0.0) | 7.1 (0.4) | 286.6 | 122.1 (10.5) | 0.1 (0.0) | 1.9 (0.2) | 124.1 |
| 900323 | 20.0 (1.3) | 10.0 (1.1) | 14.2 (1.1) | 44.3 | 76.4 (8.4) | 6.6 (0.7) | 12.0 (0.6) | 95.0 | 28.0 (2.0) | 2.5 (0.3) | 13.3 (0.5) | 43.8 | 56.8 (5.2) | 1.0 (0.0) | 6.0 (0.4) | 63.8 |
| 900329 | 77.7 (4.1) | 1.7 (0.1) | 7.5 (0.8) | 86.9 | 24.8 (3.3) | 1.1 (0.2) | 7.2 (0.8) | 33.1 | 111.1 (6.8) | 2.3 (0.2) | 29.1 (2.2) | 142.5 | 30.7 (2.7) | 1.1 (0.1) | 9.8 (1.3) | 41.6 |
| 900385 | 40.2 (3.5) | 3.6 (0.2) | 12.8 (1.0) | 56.6 | 42.0 (4.8) | 1.6 (0.1) | 22.7 (0.7) | 66.3 | 7.3 (0.6) | 0.2 (0.0) | 2.3 (0.2) | 9.8 | 10.5 (1.0) | 1.6 (0.1) | 10.4 (1.3) | 22.5 |
| 900414 | 49.8 (3.9) | 12.6 (1.0) | 5.5 (0.8) | 67.9 | 26.5 (5.3) | 1.0 (0.1) | 1.7 (0.1) | 29.2 | 45.9 (4.9) | 1.9 (0.2) | 7.2 (0.3) | 55.0 | 42.6 (4.1) | 1.7 (0.1) | 6.1 (0.4) | 50.4 |
| 900415 | 7.0 (0.6) | 11.1 (1.4) | 2.4 (0.2) | 20.5 | 2.9 (0.2) | 1.2 (0.2) | 1.0 (0.1) | 5.1 | 45.7 (3.2) | 0.2 (0.0) | 1.0 (0.1) | 46.9 | 8.9 (0.9) | 0.2 (0.0) | 0.5 (0.1) | 9.6 |
a
Mean (SE), mean concentration ± standard error for at least three independent assays per measurement. Ab, antibody.
b
Concentrations for serogroups A and C were previously published by Holder et al. (4) for the following sera: CDC1992, 900215, 900242, 900268, 900274, 900306, and 900414.
c
Total Ab concentration (total = summation of IgG + IgM + IgA) determined by summation of mean class-specific Ab concentrations.