Table 1. RNAi reveals genes essential for myosin II localization.
| Gene RNAi'd | FlyBase ID | Protein function | Fraction binucleate cells | Myosin localized: fixed cells | Myosin recruited: live cells | Myosin retained: live cells | Furrow contraction: live cells |
|---|---|---|---|---|---|---|---|
| pebble | FBgn0003041 | Rho1 GTP exchange factor | 0.44 | — | — | — | — |
| rho kinase | FBgn0026181 | Kinase; activation of myosin light chain | 0.33 | — | — | — | — |
| rho 1 | FBgn0014020 | Small GTPase | 0.38 | — | — | — | — |
| zipper | FBgn0005634 | Nonmuscle myosin II heavy chain | 0.33 | — | — | — | — |
| actin | FBgn0000042 | Structural component of cytoskeleton | 0.46 | +* | —* | —* | |
| pavarotti | FBgn0011692 | Mitotic kinesin | 0.49 | — | + | — | — |
| racGAP50c | FBgn0033881 | G-protein inactivation | 0.21 | — | + | — | — |
| chickadee | FBgn0000308 | Profilin (actin polymerization) | 0.41 | — | + | — | + |
| diaphanous | FBgn0011202 | Formin (actin polymerization) | 0.51 | — | + | — | + |
*
Live cell data is from cells treated with LatA to depolymerize actin filaments, not from cells in which actin is RNAi depleted.