TABLE 2.
Reduction of TT-induced IL-2 in the presence of cell wall extracts and GtfC-GtfD
| Donor no. | Level of cytokine (% of inhibition) after stimulation with antigensa:
|
||||||
|---|---|---|---|---|---|---|---|
| TT (10) | TT-CWP (20) | TT-GtfC (10) | TT-GtfD (10) | CWP (20) | GtfC (10) | GtfD (10) | |
| 12 | 43.6 ± 3.5 | 18.9 ± 2.2 (57) | 20.1 ± 1.4 (54) | 24.7 ± 2.0 (43) | 8.1 ± 0.9 | 7.8 ± 1.2 | 12.2 ± 1.4 |
| 15 | 26.6 ± 2.5 | 5.1 ± 0.6 (81) | 6.2 ± 1.5 (77) | 7.5 ± 1.8 (72) | <5 | <5 | <5 |
| 21 | 79.2 ± 3.4 | 26.0 ± 2.5 (67) | 37.6 ± 2.1 (53) | 46.5 ± 2.4 (41) | 12.5 ± 0.7 | 7.1 ± 1.2 | 6.9 ± 1.3 |
| 22 | 15.8 ± 1.4 | 2.1 ± 0.6 (87) | 7.8 ± 1.6 (51) | 8.2 ± 2.2 (48) | <5 | 7.3 ± 0.5 | 9.4 ± 0.9 |
a
Values represent IL-2 levels (means ± standard deviations) in supernatants generated for 5 days by T cells (2 × 105/well) in the presence of irradiated PBMC as antigen-presenting cells and various antigens. Cytokines were detected with a quantitative ELISA. Percent inhibition was calculated as 100 − value for tested antigen/value with TT alone. Cytokine levels are shown in picograms per milliliter. Numbers in parentheses after antigens are amounts in micrograms per milliliter.