TABLE 3.
Inhibition of TT-induced IFN-γ in the presence of cell wall extracts and GtfC-GtfD
| Donor no. | Level of cytokine (% of inhibition) after stimulation with antigena
|
|||||||
|---|---|---|---|---|---|---|---|---|
| TT (2)
|
TT (10)
|
|||||||
| Alone | + CWP (20) | + GtfC (10) | + GtfD (10) | Alone | + CWP (20) | + GtfC (10) | + GtfD (10) | |
| 12 | 526.1 | 329.9 (37) | 365.1 (31) | 362.6 (31) | 1,594.7 | 1,126.2 (29) | 824.7 (48) | 1,334.1 (16) |
| 15 | 399.3 | 379.1 (5) | 345.9 (13) | 346.5 (13) | 546.9 | 360.4 (34) | 382.7 (30) | 574.4 |
| 21 | 895.2 | 928.6 | 913.4 | 977.2 | 2,034.5 | 1,521.7 (25) | 1,741.7 (14) | 1,985.9 (2) |
| 22 | 330.9 | 310.7 (6) | 454.9 | 341.6 | 366.6 | 383.6 | 385.9 | 316.9 (14) |
a
Values represent average IFN-γ levels (picograms per milliliter) in supernatants generated for 5 days by T cells (2 × 105/well) in the presence of irradiated PBMC as antigen-presenting cells and various antigens. Cytokines were detected with a quantitative ELISA from quadruplicate assays with standard deviations within 5% of the mean. Percent inhibition was calculated as 100 − value for tested antigens/value for TT alone. Numbers in parentheses after antigens are amounts in micrograms per milliliter.