Detection of anti-L. donovani IgG in urine of visceral leishmaniasis patients, healthy individuals, and controls with various diseases by ELISA with crude antigen (a) or acetone-treated antigen (b). Each symbol (•) stands for a single urine sample. The horizontal dotted lines represent the cutoff values: 8.2 (a) and 7.4 (b) U. EHC, healthy controls from areas of endemicity; NEHC, healthy controls from areas of nonendemicity; M, malaria; TB, tuberculosis; OD, other diseases; n, number of samples.