Immunoblot profiles of L. donovani crude antigen polypeptides reacted with urine of VL patients and various controls. Lanes: M, molecular mass marker; St, separated polypeptides stained with Coomassie brilliant blue; 1 to 6, ELISA-positive VL; 7 to 10, ELISA-negative VL; 11 and 12, aplastic anemia; 13 and 14, healthy controls from areas of endemicity; 16 to 30, malaria; 31, tuberculosis; 32 to 36, CL; 15 and 37, casein buffer. The positions of molecular-mass markers in kilodaltons are shown on the left.