Fig. 3.

Flow cytometric analyses of the lymphoid compartment in K13 transgenic mice. (A) Spleen and lymph node cells from 8- to 10-week-old K13 and NLC mice were stained with FITC-conjugated B220 and R-phycoerythrin-conjugated Thy 1.2. antibodies. Although the B220⁺ cells are increased in K13 transgenic animals, they lack the population of B220⁺Thy1.2⁺ double-positive cells known to be present in Fas-deficient animals. (B) Splenocytes, lymphocytes, and thymocytes were stained with FITC-CD4 and RPE-CD8. CD4 and CD8 T cell subsets in the spleen, thymus, and lymph nodes were similarly represented in both K13 transgenic and NLC groups. The percentages shown are representative of more than four individual experiments in three different transgenic lines.