Fig. 2.

Role of the pgm locus in the replication of Y. pestis in naïve macrophages. Unactivated BMM were infected with KIM10+/GFP or KIM10/GFP for 20 min before gentamicin addition to the medium (0 h postinfection). At the indicated times postinfection, intracellular bacteria were recovered and enumerated by cfu assay. Fold increase values are normalized relative to the 1-h value and represent averages of triplicate samples from one representative experiment. Error bars show the standard deviations.