Fig. 5.

Curing, aggregation, and cytoduction of Ade⁺ isolates. (A) Ade⁺ isolates of strains expressing SUP35-21, -24, -26, and -27 were grown on YPD and YPD with 4 mM guanidine·HCl. Single colonies were taken from the YPD (+) and YPD plus guanidine (Gd) plates and restreaked onto YPD, along with the respective Ade^- parent strain (-). (B) Cell lysates from Ade⁺ isolates and from their Ade^- parent strain were separated by centrifugation into soluble (S) and pellet (P) fractions. Proteins in each fraction were separated by polyacrylamide gel electrophoresis and immunoblotted with antiserum specific for Sup35p. (C and D) Ade⁺ isolates were used as donors for cytoduction reactions. Cytoductions were performed as previously described (13). Recipient cells expressed the same scrambled Sup35p. Cytoductants (+), donors, and the precytoduction Ade^- recipient (-) were then streaked onto YPD. For SUP35-21, four donors containing different [PSI⁺] strains were used to test whether strain variations were maintained through cytoduction.