Fig. 3.

Gcs1-4-mediated temperature sensitivity is relieved by the presence of exogenous short-chain DAG and increased PLD activity. (A) Ten-fold serial dilutions of cells of indicated genotype (Left), carrying the WT GCS1 gene on a high-copy plasmid where indicated, were spotted on solid enriched selective medium and incubated at the indicated temperature in the presence or absence of 50 μM short-chain DAG. (B) Serial dilutions of gcs1-4 age2Δ spo14Δ triple-mutant cells harboring the indicated high-copy plasmid were treated as described in the legend of Fig. 1 A. (C) Ten-fold serial dilutions of gcs1-4 age2Δ cells harboring the indicated high-copy plasmids were treated as described in the legend of Fig. 1 A.