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. 2005 Aug 26;102(36):12777–12782. doi: 10.1073/pnas.0506156102

Fig. 4.

Fig. 4.

Exogenous DAG and overexpression of SFH2, SPO14, and SEC14-PC alleviate transport defects in gcs1-4 age2Δ cells. (A) Ratios of secreted invertase (External) to total invertase (Total) after 2 h at 37°C were determined for gcs1-4 age2Δ cells harboring the indicated genes on high-copy plasmids and also for gcs1-4 age2Δ cells growing in the presence of di8:0 DAG. Results are mean ± SD of three separate experiments, each performed in triplicate. Note that the y axis begins at a value of 0.4. (B) Endocytosis was visualized by uptake and transport of the lipophilic dye FM4-64 in gcs1-4 age2Δ cells harboring plasmids carrying the indicated genes. Samples were taken for analysis after transfer to 37°C for 30 min after the initial loading of the dye at 23°C.