Table 1. Recombinant H5-PR/8/34 viruses generated for use in this study.
| Recombinant virus
|
Amino acid position in HA1
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Full name | Abbreviation* | 36 | 83 | 86 | 120 | 155 | 156 | 189 | 212 | 223 | 263 |
| A/HK/213/03 ΔH5N1 × PR/8/34 | ΔH5N1/03 | T | A | A | N | N | A | R | K | N | A |
| A/Vietnam/1203/04 ΔH5N1 × PR/8/34 | ΔH5N1/04 | K | T | V | S | S | T | K | R | S | T |
| A/Vietnam/1203/04 ΔH5 × PR/8/34 | ΔH5/04 | K | T | V | S | S | T | K | R | S | T |
| A/Vietnam/1203/04 ΔH5S155→N, T156→A × PR/8/34 | ΔH5S155→N, T156→A/04 | T | A | V | S | N | A | K | R | S | T |
| A/Vietnam/1203/04 ΔH5S120→N × PR/8/34 | ΔH5S120→N/04 | T | A | V | N | S | T | K | R | S | T |
| A/Vietnam/1203/04 ΔH5R212→K × PR/8/34 | ΔH5R212→K/04 | T | A | V | S | S | T | K | K | S | T |
| A/Vietnam/1203/04 ΔH5S223→N × PR/8/34 | ΔH5S223→N/04 | T | A | A | S | S | T | K | R | N | A |
| A/Vietnam/1203/04 ΔH5S120→N, S155→N, T156→A × PR/8/34 | ΔH5S120→N, S155→N, T156→A/04 | T | A | A | N | N | A | K | R | S | A |
| A/HK/213/03 ΔH5N223→S × PR/8/34 | ΔH5N223→S/03 | T | A | A | N | N | A | R | K | S | A |
| A/duck/Singapore/3/97 H5 × PR/8/34 | H5/97† | T | D | V | S | N | A | K | E | S | A |
| A/duck/Singapore/3/97 H5S223→N × PR/8/34 | H5S223→N/97 | T | D | V | S | N | A | K | E | N | A |
The amino acids inserted in the HA1 of recombinant H5 viruses are shown in bold.
*
Δ multibasic amino acids in the H5 were removed by genetic engineering.
†
Wild-type A/duck/Singapore/3/97 does not have multibasic amino acids.