Table 3. H1 analysis of ΔH5 recombinant viruses with anti-H5 monoclonal antibodies.
| Monoclonal antibodies to H5 (H1 titers)
|
||||||
|---|---|---|---|---|---|---|
| Virus | VN04-6* | HK03-3† | CP24‡ | CP46‡ | CP58‡ | 406/7‡ |
| ΔH5N1/03 | 51,200 | 6,400 | 1,600 | 100 | 1,600 | 800 |
| ΔH5/04 | 12,800 | <100 | 800 | <100 | 1,600 | <100 |
| ΔH5S155→N, T156→A/04 | 3,200 | <100 | 800 | <100 | 800 | 100 |
| ΔH5S120→N/04 | 12,800 | 200 | 800 | <100 | 1,600 | <100 |
| ΔH5R212→K/04 | 12,800 | 100 | 1,600 | <100 | 6,400 | <100 |
| ΔH5S223→N/04 | 51,200 | 3,200 | 12,800 | <100 | 25,600 | <100 |
| ΔH5S120→N, S155→N, T156→A/04 | 12,800 | 1,600 | 3,200 | <100 | 1,600 | 200 |
| ΔH5N223→S/03 | 12,800 | 800 | ≤100 | <100 | 200 | ≤100 |
H1 tests were performed in microtiter plates with 0.5% chicken RBCs. Titers are the reciprocal lowest dilutions of mAbs that inhibited hemagglutination caused by 4 HAUs of virus.
Anti-HA mAb to A/Vietnam/1203/04 virus.
Anti-HA mAb to A/HK/213/03 virus.
Anti-HA mAb to A/chicken/Pennsylvania/1370/83 virus.