FIG. 3.

Identifying diminutive clonal expansions. We identified a clonal expansion of HIV-specific CD8⁺ T cells (TCRVβ21+) by using HLA class I (A0201) tetramers complexed with a Gag peptide (SLYNTVATL). The CDR3 length distributions of three fluorescence-activated cell-sorted T-cell subsets are shown. (A) CDR3 length distribution for the tetramer positive subset; (B and C) tetramer-negative and total CD8⁺-T-cell subsets, respectively. The gray vertical bar aligns the expanded CDR3 length across the panels.