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. 2025 Feb 1;19(2):315–324. doi: 10.1007/s12072-025-10778-7

Table 1.

The different sample processing procedures and analysis methods used in the studies

Reference Sample processing procedures Analysis methods
[27] 70 mM filter, and 30%, 70% Percoll density gradient centrifugation Massively parallel single-cell RNA-seq and metacell modeling
[21] 100 mm-mesh cell strainers, and 37.5%, 67.5% Percoll density gradient centrifugation Cellular indexing of transcriptomes and epitopes by sequencing and Seurat (v3.1.5)
[28] 70 μm cell strainer, 38.5% Percoll centrifuging at 325×g for 20 min Single-cell RNA-seq analysis and Seurat (v4.0.1) aiming NK1.1+ NKp46+ cells
[29] 200-gauge mesh, and 40%, 70% Percoll density gradient centrifugation Single-cell RNA-seq analysis and Seurat (v3.0) aiming CD45+ NK1.1+ NKp46+ CD3 CD19 cells

NKp46 natural killer p46