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The mRNA of IL-8 and TNF-α in human epidermal keratinocytes cocultured with T. mentagrophytes. After 0, 1, 3, and 12 h of coculture with human epidermal keratinocytes and T. mentagrophytes, mRNAs of IL-8 and TNF-α in the keratinocytes were determined by RT-PCR. IL-8 and TNF-α of mRNAs were detected after 1 to 12 h of coculture but not at 0 h of coculture.
