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. 2002 Jul;9(4):763–770. doi: 10.1128/CDLI.9.4.763-770.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — Native Pt-API-1 is present in the E/S products of adult P. tenuis worms. Serum antibodies from deer infected with P. tenuis (A) or from rats immunized with rPt-API-1 (B) specifically recognize a 27-kDa molecule in the E/S products of adult P. tenuis. E/S products were blotted and incubated with pooled sera from 11 red deer at 112 to 140 days postexperimental infection (lane 1), antibody (from white-tailed deer) affinity-purified against rPt-API-1 (lane 2), pooled sera collected from rats immunized with rDHFR (lane 3), pooled sera collected from rats immunized with rPt-API-1 (lane 4), and pooled sera collected from rats immunized with E/S products from adult P. tenuis (lane 5).