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Immunohistochemical detection of Pt-API-1 in adult and larval stages of P. tenuis. (A and C) Sections were treated with pooled sera collected from rats immunized with rDHFR. (B and D) Sections were treated with pooled sera collected from rats immunized with rPt-API-1. (A and B) Adult female; (C and D) infective L3 in situ within foot of terrestrial gastropod. M, muscle; G, gonad; C, cuticle; I, intestine.
