FIGURE 2:

Nanoparticle diffusion does not change with cell size in large multinucleate cells

(A) Images (sum projection of three middle slices) of S. pombe sid2-as cells with nuclear membrane marker Ish1-mScarlet (red). Cells were grown at steady state 30°C and treated with 0.25% DMSO or 10 µM ATP analogue 1-NM-PP1. Left to right: 6 h DMSO, 3 h 1-NM-PP1, and 6 h 1-NM-PP1. Scale bar is 5 µm. (B) Cell length, (C) number of nuclei (mean ± STD of replicate experiments; N_CELLS ≥ 140 per condition from three biological replicates), and (D) cytGEMs diffusion coefficients (mean ± STD of replicate experiments; N_GEMS ≥ 5546 per condition from four biological replicates) for sid2-as cells (one-way ANOVA, *** - p < 0.001, **** - p < 0.0001).