TABLE 1.
Key resources used in this study.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Figure | Additional Information |
|---|---|---|---|---|---|
| Genetic reagent (S. pombe) | Ish1-mScarlet | Chang Lab collection | FC 3318 | 1 | h- ade6<<mCherry-psy1 ish1-GFP:kanMX ura4-D18 |
| Genetic reagent (S. pombe) | Ish1-mScarlet, cdc25 mutant | This manuscript | FC 3339 | 1 | cdc25-22 ade6<<mCherry-psy1 ish1-GFP:kanMX |
| Genetic reagent (S. pombe) | Ish1-mScarlet, wee1 mutant | This manuscript | FC 3340 | 1 | wee1-50 ish1-GFP:kanMX |
| Genetic reagent (S. pombe) | cytGEMs | Chang Lab collection | FC 287 | 1 | h- pREP41X-PfV-Sapphire leu1-32 |
| Genetic reagent (S. pombe) | cytGEMs, cdc25 mutant | This manuscript | FC 3341 | 1 | h+ cdc25-22, pREP41X-PfV-Sapphire leu1-32 |
| Genetic reagent (S. pombe) | cytGEMs, wee1 mutant | This manuscript | FC 3342 | 1 | h- wee1-50, pREP41X-PfV-Sapphire leu1-32 hist? |
| Genetic reagent (S. pombe) | cytGEMs, cdc2 mutant | This manuscript | FC 3343 | 1 | h90 cdc2-asM17, pREP41X-PfV-Sapphire, leu1-32, ura4-D18 |
| Genetic reagent (S. pombe) | cytGEMs, sid2 mutant, ish1-mScarlet | This manuscript | FC 3344 | 2 | h+ sid2-as ish1:mScarlet-I:hphMX6 ade6-M210, pREP41X-PfV-Sapphire, leu1-32 ura4-D18 |
| Genetic reagent (S. pombe) | cytGEMs, cdc11 mutant | This manuscript | FC 3345 | 2 | h- cdc11-119, pREP41X-PfV-Sapphire leu1-32 |
| Genetic reagent (S. pombe) | Rps2-GFP | Chang Lab collection | FC3209 | 3 | h- rps2-GFP::kanR leu1-32 ura4-D18 ade6-210 |
| Genetic reagent (S. pombe) | Rps2-GFP, cdc25 mutant | This manuscript | FC 3346 | 3 | cdc25-22 rps2-GFP::kanR leu1-32 ura4-D18 |
| Genetic reagent (S. pombe) | Rps2-GFP, wee1 mutant | This manuscript | FC 3347 | 3 | wee1-50 rps2-GFP::kanR leu1-32 ade6-210 |
| Genetic reagent (S. pombe) | car2 mutant | This manuscript | FC 3348 | 4 | car2∆::kanMX4 arg1-230 lys3-37 |
| Genetic reagent (S. pombe) | car2 mutant, cdc25 mutant | This manuscript | FC 3349 | 4 | cdc25-22 car2∆::kanMX4 arg1-230 lys3-37 |
| Genetic reagent (S. pombe) | car2 mutant, wee1 mutant | This manuscript | FC 3350 | 4 | wee1-50 car2∆::kanMX4 arg1-230 lys3-37 |
| Chemical compound/drug | YES 225 Media | Sunrise Science Production | #2011 | 3 | |
| Chemical compound/drug | Edinburgh Minimum Media (EMM) | MP Biomedicals | #4110-32 | 1-4 | |
| Chemical compound/drug | Agar | ||||
| Chemical compound/drug | Histidine | Sigma-Aldrich | #H8000 | 1-3 | |
| Chemical compound/drug | Uracil | Sigma-Aldrich | #U0750 | 1-3 | |
| Chemical compound/drug | Adenine | Sigma-Aldrich | #A9126 | 1-3 | |
| Chemical compound/drug | Thiamine | Sigma-Aldrich | #T4625 | 1, 2 | |
| Chemical compound/drug | DMSO | Thermo Fisher Scientific | #67-68-5 | 1, 2 | |
| Chemical compound/drug | 1-NM-PP1 | Thermo Fisher Scientific | #50-203-0494 | 1, 2 | |
| Chemical compound/drug | Agarose | Invitrogen | #16500500 | 3 | |
| Chemical compound/drug | Dulbecco's Phosphate Buffer Saline | Thermo Fisher Scientific | 14190144 | 3 | |
| Chemical compound/drug | 4% formaldehyde (methanol-free) | Thermo Fisher Scientific | #28,906 | 3 | |
| Chemical compound/drug | RNAse A | Thermo Fisher Scientific | #EN0531 | 3 | |
| Chemical compound/drug | Fluorescin isothiocyanate isomer I | Sigma | #F7250 | 3 | |
| Chemical compound/drug | Light arginine (L-ARGININE:HCL–Unlabeled) | Cambridge Isotope Laboratories | #ULM-8347-PK | 4 | |
| Chemical compound/drug | Heavy arginine (L-ARGININE:HCL(13C6,99%)) | Cambridge Isotope Laboratories | #CNLM-2265-H-0.25 | 4 | |
| Chemical compound/drug | Light lysine (L-LYSINE:2HCL–Unlabeled) | Cambridge Isotope Laboratories | #ULM-8766-PK | 4 | |
| Chemical compound/drug | Heavy lysine (L-LYSINE:2HCL(4,4,5,5-D4,96-98%)) | Cambridge Isotope Laboratories | #DLM-2640-0.5 | 4 | |
| Chemical compound/drug | Iodoacetamide | Sigma | #I1149-5G | 4 | |
| Chemical compound/drug | TPCK-treated trypsin | Worthington | #LS003740 | 4 | |
| Chemical compound/drug | Sep-Pak 50 mg C18 column | Waters | ##054955 | 4 | |
| Software, algorithm | µManager v. 1.41 | Edelstein et al., 2010; Edelstein et al., 2014 | 1-3 | ||
| Software, algorithm | Mathworks | Mathworks | 2018a | 1, 2 | |
| Software, algorithm | Python | Drake Jr. and Van Rossum, 1995 | 3.8.8 | 3, 4 | |
| Software, algorithm | Prism | GraphPad | Version 9.4.1 | 1-3 | |
| Software, algorithm | FIJI ImageJ | Schindelin et al., 2012 | 1-3 | ||
| Software, algorithm | MaxQuant (v2.4.2) | 4 | |||
| Other | µ-Slide VI 0.4 channel slide | Ibidi | #80606 | 1, 2 |