Table 1.
Interaction of Y361 in different conformations demonstrating the switch in interaction from R439 toward E386
| Conformation | Ligand | Interaction partner of Y361 | PDB ID |
|---|---|---|---|
| Outward open | Apo | R439 | 8ET6 |
| Metformin | R439 | 8JTS | |
| Outward occluded | Metformin | R439 | 8JTT |
| Inward occluded | Metformin | R439 | 8JTV |
| Inward partially open | Apo | E386 | 8JTW |
| Apo | None | 8JTY | |
| Inward open | Apo | None | 8JTX |
| Apo | E386 | 8SC1 | |
| Metformin | E386 + substrate | 8SC4 | |
| Fenoterol | E386 + substrate | 8SC3 | |
| Thiamine | E386 | 8SC6 |
Available cryo-EM structures resolving OCT1 (apo or substrate bound, (21, 22, 23)) were analyzed for interaction partners of the Y361 residues using the interaction analyses of the PDB database (www.rcsb.org).