Figure 2.

In Vitro binding affinity of MJ compounds. All compounds were competed against ³H-diprenorphine in binding assays against all three human opioid receptors expressed in CHO cells. Positive control compounds (naloxone for MOR and DOR, U50, 488 for KOR) were included to validate the assay. The concentration–response curves shown are summary curves with the mean value ± SEM from N ≥ 3 independent experiments for each receptor as noted. The K_I values in the table below were calculated separately for each independent experiment and reported as the mean ± SEM.