Table 5.

– Uratylation sites (∗ = +140 Da adduct in lysine (K) residues) were identified in HUVEC secretome and lysate after 1:30 and 24h exclusively in urate treated samples (50–200 μM). PTM analysis was performed with MaxQuant using raw files from initial proteomic experiments.

Protein name	Gene name	Sequence	Type of sample/incubation time
Serine/arginine repetitive matrix protein 2	SRRM2	TKQPSSPYEDKDKDKK∗EK∗SATRPSPSPERSS	Secretome – 24 h, Lysate – 24 h
Matrix-remodeling-associated protein 5	MXRA5	MRWFLEWDAKSRGILK∗CK∗K∗DK∗AYEGGQLCAM	Lysate – 24 h
Rho-associated protein kinase 2	ROCK2	EKRQLQERFTDLEKEK∗SNMEIDMTYQLKVIQ	Secretome – 1:30 and 24 h
Zinc finger CCCH domain-containing protein 15	ZC3H15	SKKAEQKKKEKIIEDK∗TFGLK∗NK∗K∗GAKQQKF	Lysate – 1:30 and 24 h
Tubulin polyglutamylase	TTLL6	ICRKDLLARNMSRMLK∗MFPKDFRFFPRTWCL	Secretome 1:30, Lysate – 24 h
Sorting nexin-5	SNX5	AAFRKNLIEMSELEIK∗HARNNVSLLQSCIDL	Lysate – 24 h
Myosin-15	MYH15	TNQVREGTKNLTEMEK∗VK∗K∗LIEEEKTEVQVT	Lysate – 24 h