TABLE 1.
Oligonucleotide sequences of primers used in this study
| Primer name | Oligonucleotide sequence (5′→3′)a | Reference |
|---|---|---|
| PCR amplification for groESL | ||
| EEgro1F | 5-GAG-AGA-TGC-TTA-TGG-TAA-GAC-3 | 1 |
| EEgro2R | 5-CAG-CGT-CGT-TCT-TAC-TAG-GAA-C-3 | 1 |
| Sequence primers for groESL | ||
| SQ3F | 5-ATT-AGC-AAG-CCT-TAT-GGG-TC-3 | This study |
| SQ4R | 5-CTT-TAG-GCT-ATC-AAG-AGA-TG-3 | This study |
| SQ4F | 5-CAT-CTC-TTG-ATA-GCC-TAA-AG-3 | This study |
| SQ5F | 5-TCA-GTG-TGT-GAA-GGA-AGT-TG-3 | This study |
| SQ6R | 5-TGC-TTC-CTA-TGT-TCT-TAT-CG-3 | This study |
| PCR amplification of gltA fragments | ||
| F4b | 5-CCG-GGT-TTT-ATG-TCT-ACT-GC-3 | 8 |
| F1b | 5-GAT-CAT-GAR-CAR-AAT-GCT-TC-3 | This study |
| EHR-CS779R | 5-GCN-CCM-CCA-TGM-GCT-CG-3 | 14 |
| HG-1085R | 5-ACT-ATA-CCK-GAG-TAA-AAG-TC-3 | 8 |
| gltA-specific primer for the Genome Walker method | ||
| PLATYS-SPF | 5-CTG-CCG-GAA-CAG-AGC-TAT-TC-3 | This study |
| PLATYS-SPR1 | 5-TCG-GAT-GAC-AGA-GCA-TAG-TG-3 | This study |
| PLATYS-SPR2 | 5-TTG-CTC-GAA-CAC-TGT-GTC-TG-3 | This study |
| A. platys-specific primers | ||
| PLA-HS475F | 5-AAG-GCG-AAA-GAA-GCA-GTC-TTA-3 | This study |
| PLAT-HS1198R | 5-CAT-AGT-CTG-AAG-TGG-AGG-AC-3 | This study |
| PLA-CSM136F | 5-TTG-CAA-AAA-GTA-AGC-GGA-GC-3 | This study |
| PLA-CS1359R | 5-AAC-CAC-AGG-CTT-ATG-ACA-AC-3 | This study |
a
K, G or T; R, G or A; M, C or A; N, G, C, A, or T.