Table 1.

Inhibition of focus formation by prostate cancer cell line PC3 expressing U94 protein.

Cell line	No. of foci/75 cm2flask
Parental PC3 cell line	80
Vector transfected PC3 cell line	69
PC3 cell line stably expressing U94	2

Clonal PC3 cell lines, transfected with plasmid containing U94 gene and geneticin (G418) resistant cassette, were used for studies. PC3 cell line transfected with vector cassette or parental PC3 cell line served as controls. G418 resistant clones were sub-cultured and grown to confluence. Focus formation was detected as dense foci of actively growing and refractive cells. The experiment was performed in duplicate, and the average number of foci was reported. Notice that stable expression of U94 drastically inhibited focus formation.