Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) |
trpγ1 | Bloomington Drosophila Stock Center | BDSC:64311 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | trpγG4 | Bloomington Drosophila Stock Center | BDSC:64313 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | trpγ1,UAStrpγ/CyO | Akitake et al., 2015 | Provided by Dr. C. Montel | |
| Genetic reagent (D. melanogaster) | trpγ1;g(trpγ) | Akitake et al., 2015 | Provided by Dr. C. Montel | |
| Genetic reagent (D. melanogaster) | trpA11 | Bloomington Drosophila Stock Center | BDSC:26504 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | amo1 | Watnick et al., 2003 | Provided by Dr. C. Montel | |
| Genetic reagent (D. melanogaster) | trpml2 | Bloomington Drosophila Stock Center | BDSC:42230 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | trp343 | Bloomington Drosophila Stock Center | BDSC:25082 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | pyx3 | Lee et al., 2005 | Dr. Y. Lee | |
| Genetic reagent (D. melanogaster) | wtrwex | Bloomington Drosophila Stock Center | BDSC:59038 | Provided by Dr. C. Montel |
| Genetic reagent (D. melanogaster) | trpl29134 | Bloomington Drosophila Stock center | BDSC:29134 | |
| Genetic reagent (D. melanogaster) | iav3621 | Bloomington Drosophila Stock center | BDSC:24768 | |
| Genetic reagent (D. melanogaster) | pain2 | Tracey et al., 2003 | Provided by Dr. S. Benzer | |
| Genetic reagent (D. melanogaster) | nan36a | Kim et al., 2003 | Provided by Dr. C. Kim | |
| Genetic reagent (D. melanogaster) | diLP2-GAL4 | Korea Drosophila Resource Center | KDRC: 200 | |
| Genetic reagent (D. melanogaster) | Dh44-GAL4 | Korea Drosophila Resource Center | KDRC: 2543 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | UAS-mCD8::GFP | Bloomington Drosophila Stock Center | BDSC: 5130 | |
| Genetic reagent (D. melanogaster) | UAS-Kir2.1 | Bloomington Drosophila Stock Center | BDSC: 6596 | |
| Genetic reagent (D. melanogaster) | UAS-trpγRNAi | Vienna Drosophila Resource center | Transformant ID107656 | |
| Genetic reagent (D. melanogaster) | r4-GAL4 | Korea Drosophila Resource Center | KDRC: 2166 | |
| Genetic reagent (D. melanogaster) | cg-GAL4 | Bloomington Drosophila Stock Center | BDSC: 7011 | Provided by Dr. S. Hyun |
| Genetic reagent (D. melanogaster) | Myo1A-GAL4 | Bloomington Drosophila Stock Center | BDSC: 67057 | Provided by Dr. S. Hyun |
| Genetic reagent (D. melanogaster) | UAS-AMPKTD | Korea Drosophila Resource Center | KDRC:10099 | |
| Genetic reagent (D. melanogaster) | UAS-bmm | Bloomington Drosophila Stock Center | BDSC: 76600 | |
| Genetic reagent (D. melanogaster) | Dh44Mi | Bloomington Drosophila Stock Center | BDSC: 24345 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | Dh44R1Mi | Bloomington Drosophila Stock Center | BDSC: 23517 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | Dh44R2Mi | Bloomington Drosophila Stock Center | BDSC: 29129 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | Dh44R1-GAL4 | Korea Drosophila Resource Center | KDRC: 2734 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | Dh44R2-GAL4 | Bloomington Drosophila Stock Center | BDSC: 66865 | Provided by Dr. Y. Kim |
| Genetic reagent (D. melanogaster) | Dh44R2RNAi | Korea Drosophila resource center | KDRC: 5121 | |
| Genetic reagent (D. melanogaster) | yw;+;bmm::GFP | Men et al., 2016 | Provided by Dr. Kaeko Kamei | |
| Genetic reagent (D. melanogaster) | Dh44-LexA | Korea Drosophila Resource Center | KDRC: 2776 | |
| Genetic reagent (D. melanogaster) | Dh44R2-LexA | Korea Drosophila Resource Center | KDRC: 6616 | |
| Genetic reagent (D. melanogaster) | LexAop-mCherry | Korea Drosophila Resource Center | KDRC: 1247 | |
| Antibody | Mouse anti-GFP (mouse monoclonal) | Molecular probe | Cat # A11120 RRID:AB_221568 |
1:1000 (1 µL) |
| Antibody | Rabbit anti-DsRed(rabbit polyclonal) | Takara | Cat # 632496 RRID:AB_ 10013483 |
1:1000 (1 µL) |
| Antibody | Goat anti-mouse Alexa Fluor 488 | Thermo Fisher/ Invitrogen |
Cat # A11029 RRID:AB_2534088 |
1:200 (1 µL) |
| Antibody | Goat anti-mouse Alexa Fluor 568 | Thermo Fisher/ Invitrogen |
Cat # A11004 RRID:AB_2534072 |
1:200 (1 µL) |
| Antibody | Goat anti-rabbit Alexa Fluor 488 | Thermo Fisher/ Invitrogen |
Cat # A11034 RRID:AB_2576217 |
1:200 (1 µL) |
| Antibody | Goat anti-rabbit Alexa Fluor 568 | Thermo Fisher/ Invitrogen |
Cat # A11036 RRID:AB_10563566 |
1:200 (1 µL) |
| Antibody | Rabbit anti-Dh44 (rabbit polyclonal) | 1:500 Provided by Dr.J.A. Veenstra |
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| Commercial assay or kit | Glucose (HK) Assay Kit | Sigma-Aldrich | Cat # GAHK-20 | |
| Commercial assay or kit | Glucose (HK) Assay reagent | Sigma-Aldrich | Cat # G3293 | |
| Commercial assay or kit | The Pierce BCA protein assay kit | Thermo Fischer Scientific | Cat # 23225 | |
| Commercial assay or kit | LiquiColor triglyceride test kit | Stanbio Laboratory | Cat # 2100–225 | |
| Chemical compounds or drug | Trehalase from porcine kidney | Sigma-Aldrich | Cat # 9025-52-9 | |
| Chemical compound or drug | Amyloglucosidase from Aspergillus | Sigma-Aldrich | Cat # 9032-08-0 | |
| Chemical compound or drug | Triglyceride mix | Sigma-Aldrich | Cat #17810-1AMP-S | |
| Chemical compound or drug | Lipase from Candida rugosa | Sigma-Aldrich | Cat # L1754 | |
| Chemical compound or drug | Metformin | Sigma-Aldrich | Cat # D150959 | |
| Chemical compound or drug | Hexanoic acid | Sigma-Aldric | Cat # W255912 | |
| Chemical compound or drug | Sucrose | Sigma-Aldrich | Cat # 57-50-1 | |
| Chemical compound or drug | Glycerol | Sigma-Aldrich | Cat # G5516 | |
| Chemical compound or drug | Nile red | Sigma-Aldrich | Cat # N3013 | |
| Software, algorithm | Origin Pro Version | OriginLab corporation | RRID:SCR_002815 | https://www.originlab.com/ |
| Software, algorithm | Graphpad Prism | GraphPad | RRID:SCR_002798 | https://www.graphpd.com/ |
| Software, algorithm | Fiji(ImageJ-win64) | Fiji | RRID:SCR_002285 | https://fiji.sc |