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Annexin V binding to lymphocytes. Annexin V binding to the cell surfaces of PI-negative PBMCs (A) and of CD4-PE- or CD8-PE-stained T lymphocytes obtained at entry and cultured overnight (B) was measured by flow cytometry using a wide forward and side scatter gate. The number of samples in each group is indicated at the base of each bar. The error bars indicate the standard error of the mean. ∗, P < 0.05 (Mann-Whitney U test); ∗∗∗, P < 0.001 (Wilcoxon rank test).
