TABLE 1.
Probes hybridizing to colony blots of CDEC isolates
| Target gene | Properties of target gene | Description of probe (reference) | Positive controls | No. (%) of CDEC strains carrying target gene
|
||
|---|---|---|---|---|---|---|
| From children with diarrhea | From healthy controls | Total | ||||
| hlyA | Hemolysin | 2.2-kb HindIII fragment from pANN215 (6) | Uropathogenic E. coli strain 536, CDEC strain A70.1 | 15 (100) | 8 (100) | 23 (100) |
| papA | Adhesin | 3-kb HindIII fragment from pRHU845 | 536, A70.1 | 15 (100) | 8 (100) | 23 (100) |
| cnfI | Cytotoxic necrotizing toxin 1 | 950-bp BamHI fragment from pSE266 (4) | 536, A70.1 | 11 (73.3) | 5 (50) | 16 (69.6) |
| stx1 and stx2 | Shiga toxins 1 and 2 | pNN110-18 (SmaI/PstI), pJN37-19 (BamHI) (17) | Enterohemorrhagic E. coli strain EDL933 | 1 (6.5) | 1 (12.5) | 2 (8.7) |
| CVD432, astA, pet | Enteroaggregative E. coli plasmid (pAA) probe; enteroaggregative heat-stable enterotoxin; and enteroaggregative plasmid-encoded enterotoxin | 0.9-kb EcoRI-PstI fragment (1); 250-bp NruI-ClaI fragment from pJPN61 (15); 2.5-kb MluI-PstI fragment from pJPN205 (5) | Enteroaggregative E. coli strain 042 | 2 (13.0) | 0 (0) | 2 (8.7) |
| intI1 | Integrase associated with class 1 integrons | 1,175-bp RsaI-BamHI fragment from Tn21 (24) | K-12 strain C600 carrying plasmid R100 | 10 (66.7) | 4 (50.0) | 14 (60.9) |
| intI2 | Integrase associated with class 2 integrons | 1,560 bp SphI-HpaI fragment from Tn7 were used as intI1 and intI2 probes (26). | K-12 strain C600 carrying plasmid R483 | 1 (6.7) | 0 (0) | 1 (4.3) |