FIG. 1.

Immune reactivity of human sera to the recombinant gB expressed in bacteria. The recombinant protein gB was expressed in bacteria as a fusion protein with a polyhistidine tract at its N terminus. The proteins were separated by denaturing SDS-PAGE, transferred to a nitrocellulose sheet, and then allowed to react with distinct human sera (lanes C to K) or to the mouse antipolyhistidine antibody (lane L). In lanes A and B, P1 serum was tested against lysates of bacteria transformed with the plasmid pTrc-HisC containing no insert and the plasmid pTr-gB-rec in the absence of IPTG induction respectively, as negative controls. Numbers on the left of gel are in kilodaltons. The star to the right of gel indicates the position of recombinant gB. NA, not applicable.