TABLE 1.

Results of HHV-7-specific peptide-based ELISA with six reference HHV-7-seronegative sera

Tested serum	Antibody titer		Intra-assay variabilitya				Interassay variabilitya
	HHV-6	HHV-7	No.b	OD			No.	OD
				Mean	SD	CV (%)		Mean	SD	CV (%)
None (blank reaction)	NAc	NA	8	0.089	0.063	7.1	14	0.086	0.015	17.1
N1	>1,280	<10	4	0.199	0.019	9.5	5	0.207	0.018	8.8
N2	>1,280	<10	4	0.174	0.014	8.3	3	0.160	0.020	13.8
N3	>1,280	<10	3	0.182	0.006	3.3	2	0.177	0.006	3.6
N4	>1,280	<10	3	0.173	0.019	11.0	2	0.173	0.019	11.0
N5	>1,280	<10	4	0.183	0.024	13.2	2	0.177	0.008	4.8
N6	>1,280	<10	3	0.217	0.097	44.7	2	0.162	0.077	47.0

^aThe variability of OD signal was studied by comparing the values obtained from different wells of the same microplate within the same assay (intra-assay variability) and the values obtained in independent experiments (interassay variability).

^bNumber of independent wells (intra-assay variability) or independent assays (interassay variability).

^cNA, not applicable.