TABLE 2.

Results of peptide ELISA with eight reference HHV-7-positive human sera

ELISA parameter	Tested serum
	P1	P2	P3	P4	P5	P6	P7	P8
Intra-assay variabilitya
No.b	2	2	4	2	2	4	4	2
Mean OD	1.107	0.563	0.960	0.598	0.692	1.009	0.642	0.885
SD	0.084	0.039	0.069	0.042	0.053	0.060	0.021	0.074
CV (%)	7.6	7.0	7.1	6.9	7.6	5.9	3.2	8.3
Interassay variabilitya
No.b	8	13	11	7	7	7	7	8
Mean OD	1.142	0.489	1.188	0.669	0.698	1.091	0.717	0.866
SD	0.069	0.072	0.122	0.066	0.098	0.093	0.045	0.061
CV (%)	6.0	14.6	10.3	9.8	14.1	8.5	6.3	7.0
OD in absence of H7GB129-152
No peptide	0.052	0.085	0.075	0.062	0.063	0.061	0.053	0.044
Irrelevant peptidec	0.132	0.164	0.167	0.152	0.151	0.141	0.158	0.144
% Reduction of OD following preincubation with cell lysates
Uninfected SupT1	5	29	0	11	20	0	11	17
HHV-7-infected SupT1	40	58	56	52	50	43	40	56
Uninfected Sf21	11	22	0	0	17	0	0	0
gB-expressing Sf21	46	66	30	26	57	26	60	42
Uninfected MT4	20	2	4	10	13	7	11	0
HHV-6-infected MT4	11	0	11	3	19	6	0	0
Uninfected HF	18	0	13	0	9	13	0	6
HCMV-infected HF	20	5	8	0	11	2	3	0
% Reduction of OD following pre-incubation with H7GB129-152 at final concn (μg/ml) of:
5	27	30	32	48	14	23	37	24
50	75	45	66	61	70	67	63	61

^aSee footnote^a of Table 1.

^bSee footnote^b of Table 1.

^cPeptide derived from the HHV-8 glycoprotein K8.1.