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Design of forward primer and probe for the identification of Borrelia species in the LightCycler. The forward primer (OspA iLC) labeled with LC Red 640 is incorporated into the first strand of DNA amplicon (A), and the fluorescein-labeled probe (Probe Ba2) binds to this DNA strand (B). The proximity of the two fluorochromes allows induction of LC Red 640 fluorescence by FRET. Due to the surplus of probes, the FRET signal depends directly on the amount of amplicon formed.
