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Detection limit of PCR assay for detection of OspA determined with serial dilutions of VS461 genomic DNA. The dilutions were utilized in doublets or triplets. All samples were amplified simultaneously. The DNA in the sample corresponded to 1,000, 100, 10, and 1 spirochete of B. afzelii (VS461). As a negative control, water was added to the reaction mixture instead of template.
