TABLE 1.

Primers used for detection of 23S rRNA gene point mutations in H. pylori isolates from patients in China

Sequence detected or purpose	Primer	Nucleotide sequence	Location (size [bp]) of PCR producta	Annealing temp (°C)
23S rRNA	5gb	5′-TCAGGGTGATGGACTGC-3′	1027–2665 (1,639)	56
	ZGE23b	5′-CACAGGCCAGTTAGCTA-3′
A2142 G	5gb	5′-TCAGGGTGATGGACTGC-3′	1027–2530 (1,504)	60
	DP4b	5′-AGGTCCACGGGGTCTTC-3′
A2143G	5gb	5′-TCAGGGTGATGGACTGC-3′	1027–2531 (1,505)	59
	DP5b	5′-AAGGTCCACGGGGTCTC-3′
A2142C	5gb	5′-TCAGGGTGATGGACTGC-3′	1027–2535 (1,509)	65
	DP6	5′-AGTAAAGGTCCACGGGGTCTTG-3′
Sequencing	2451-F	5′-TCAACCAGAGATTCAGT-3′	2451–2665 (215)	50
	ZGE23b	5′-CACAGGCCAGTTAGCTA-3′

^aPrimer positions are according to the 23S rRNA sequence published by Taylor and colleagues (13).

^bThe primer name and sequence are the same as those reported by Taylor et al. (13).