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. Author manuscript; available in PMC: 2005 Sep 9.
Published in final edited form as: J Biol Chem. 2004 Sep 22;279(51):53435–53441. doi: 10.1074/jbc.M409421200

Fig. 3. Fluorescence localization of wild type and GG4 mutants G202L and G206L of yeast CTR3-GFP.

Fig. 3

Colonies of yeast transformants of the same constructs used in complementation experiments were spotted directly on glass coverslips for fluorescence microscopy. Bright-field and corresponding fluorescence images are shown. A, plasma membrane localization of wild type yCTR3-GFP fusion protein. GG4 mutants G202L (panel B) and G206L (panel C) are trapped in a perinuclear intracellular compartment as revealed by DAPI staining of nuclear DNA and a ring at the periphery of the cell. Accumulation of GFP-tagged mutant proteins in both regions is indicative of ER localization in yeast. Smaller DAPI-stained points represent mitochondrial DNA.