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. Author manuscript; available in PMC: 2005 Sep 9.
Published in final edited form as: J Biol Chem. 2004 Sep 22;279(51):53435–53441. doi: 10.1074/jbc.M409421200

Fig. 6. Fluorescence localization of tryptophan-scanning mutants of the third transmembrane domain of yeast CTR3-GFP fusion.

Fig. 6

Thirteen separate constructs were synthesized by subcloning the wild-type yCTR3 into the p423GPD vector and performing site-directed mutagenesis to replace each amino acid position from 196 to 208 with tryptophan. Mutants were transformed, picked from minimal dextrose selective plates, and subjected to fluorescence microscopy. A, class of six mutants exhibited localization that was identical to wild type. B, second class of mutants revealed abnormal localization to intracellular compartments.