TABLE 3.
Heterogeneity of eight GBS MSs and subtypes in regions of 3′ end of cpsD and 5′ end of cpsE
| Gene and sitesa | Nucleotide
|
Specificity | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Ia | Ib | II and III-4e | III | IV | V | VI | VII | ||
| cpsD | |||||||||
| 62 | G | A | Gb | A | A | A | A | G | Ia, II, VII |
| 78–86 | − in Ia-2c and + in Ia-1c | − | − in II-2b and + in II-1d | − in III-2; + in III-1 and in III-3e | + | + | − | + | See text |
| cpsD-cpsE spacer | |||||||||
| 138 | G | G | G | G | G | Af | G | G | V |
| 139 | G | G | G | A in III-2; G in III-1 and III-3 | G | G | G | G | III-2 |
| 144 | T | T | T | G in III-2; T in III-1 and III-3 | T | T | T | T | III-2 |
| cpsE | |||||||||
| 198 | A | C | Ab | A | C | Cf | A | A | Ib, IV, V |
| 204 | G | G | G | A in III-2 and III-3; G in III-1 | G | G | G | G | III-2, III-3 |
| 211 | T | T | T | T | T | T | G | T | VI |
| 218 | C | C | C | C | C | C | T | C | VI |
| 240 | T | T | T | T | T | T | C | T | VI |
| 249 | T | C | Tb | T | C | Cf | T | T | Ib, IV, V |
| 300 | C | C | C | T in III-2; C in III-1 and III-3 | C | C | C | C | III-2 |
| 321 | C | C | C | T in III-1; C in III-2 and III-3 | C | C | C | C | III-1 |
| 419 | T | C | Tb | T | T | T | T | T | Ib |
| 429 | A | T | Ab | T | T | T | T | A | Ia, II, VII |
| 437 | C | C | C; T in III-4 | C | C | C | C | T | VII, III-4 |
| 457 | T | A | Cb | A | A | A | A | C | Ia, II, VII |
| 466 | G | G | G | G | A | G | G | A | IV |
| 486 | G | A | A | G in III-3; A in III-2 and III-1 | A | A | A | A | Ia, III-3 |
| 602 | G | G | Ab | G | G | G | G | A | II, VII |
| 606 | T | T | T | T | T | T | C | T | VI |
| 627 | T | C | C | C | C | C | C | C | Ia |
| 636 | C | T | T | C in III-1; T in III-2 and III-3 | T | T | T | T | Ia, III-1 |
| 645 | C | T | Cb | C | T | T | C | C | Ib, IV, V |
| 803 | A | A | A | A | A | A | T | A | VI |
| 971 | C | T | T | C | C | C | T | T | Ia, III, IV, V |
| 1026 | A | G | G | G in III-2 and III-1; A in III-3 | A | A | G | G | Ia, III-3, IV, V |
| 1044 | T | T | T | T | T | T | C | T | VI |
| 1173 | A | G | A | A | A | A | A | A | Ib |
| 1194 | C | C | C | A | A | C | A | C | III, IV, VI |
| 1251 | G | G | G | G | G | G | A | G | VI |
| 1278 | A | A | A | A | A | G | A | A | V |
Numbering start point 1 refers to start point 1 of GenBank accession number AF332908 (for serotype IV reference strain 3139). A repetitive sequence (TTACGGCGA) is found at in gene cpsD at positions 78 to 86.
One serotype II strain, as determined by CS, has mutations at the nine sites (see text).
Repetitive sequence is present (+) in Ia-1 and absent (−) in Ia-2 (see text).
Repetitive sequence is present (+) in II-1 and absent (−) in II-2 (see text).
Repetitive sequence is present (+) in III-1 and III-3 and absent (−) in III-2; it is variable in III-4 (see text).
The nucleotides at positions 138, 198, and 249 in one serotype V reference strain, as determined by CS (Prague 10/84), are identical to the corresponding nucleotides in GenBank (GenBank accession number AF349539); the nucleotides are G, A, and T, respectively. In another serotype V reference strain as determined by CS (CJB 111) and all the other sequenced serotype V strains, as determined by CS, the nucleotides are identical; the nucleotides are A, C, and C, respectively.