TABLE 5.

Algorithm for GBS MS identification by PCR and sequencing

Amplification primer pairsa	PCR product size (bp)	Interpretation
GBS identification primer pairs
Sag59-Sag190	196	GBS (S. agalactiae)
DSF2-DSR1	276	GBS (S. agalactiae)
GBS MS identification by MS-specific PCR
IacpsHS1-cpsIA	354	Serotype Ia
IbcpsIS-IbcpsIA1	523	Serotype Ib
IIIcpsHS-cpsIA	641	Serotype III
IVcpsHS1-IVcpsMA	379	Serotype IV
VcpsHS2-VcpsMA	374	Serotype V
VIcpsHS1-VIcpsIA	360	Serotype VI
GBS MS identification by sequencing
cpsES3-cpsGA1	790	See Fig. 1b
cpsES3-cpsFA	450	See Fig. 1b
cpsFS-cpsGA1	423	See Fig. 1b

^aSee Table 2 for primer sequences.

^bSee Fig. 1 to identify serotypes according to the sequence heterogeneity.