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Regression Analysis of qPCR and Flow Cytometry. Regression analysis for the logarithmic values of normalized OT-1 transgene copy (defined as the number of OT-1 copies/10,000 β-actin copies) and actual percentage of OT-1 cells by flow cytometry. OT-1 dilutions of 100%, 4%, 1,3%, 0.433%, 0.144%. 0.048%, and 0.016% were used for the analysis and the R2 value was calculated to be 0.9458.
