TABLE 1.
PCR primers used in this study
| Designation | Location | Sequence (5′ to 3′) | Target gene | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| V1 | 213–230 | AGT-TAA-TGT-GGT-GGC-GAA | |||
| V5 | 1013–1029 | GAC-TCT-TCC-ATC-TGC-CG | stx1 | 817 | 16 |
| V3 | 289–306 | TTC-GGT-ATC-CTA-TTC-CCG | |||
| V4 | 745–762 | TCT-CTG-GTC-ATT-GTA-TTA | stx2 | 474 | 16 |
| EA-1 | 1846–1865 | AAA-CAG-GTG-AAA-CTG-TTG-CC | |||
| EA-2 | 2280–2299 | CTC-TGC-AGA-TTA-ACC-TCT-GC | eaeA | 454 | This study |
| EP-1 | 2773–2793 | AAT-GGT-GCT-TGC-GCT-TGC-TGC | |||
| EP-2 | 3076–3096 | GCC-GCT-TTA-TCC-AAC-CTG-GTA | bfpA | 324 | 9 |
| PerAS | 522–541 | TGT-CAT-CCT-TAG-TGC-TTC-AT | |||
| PerAAS | 856–875 | GGC-AAT-GTT-CCT-TGT-GTA-AT | perA | 354 | This study |
| EAST-1S | 63–82 | GCC-ATC-AAC-ACA-GTA-TAT-CC | |||
| EAST-1AS | 149–168 | GAG-TGA-CGG-CTT-TGT-AGT-CC | astA | 106 | This study |
| AggRks1 | 100–120 | GTA-TAC-ACA-AAA-GAA-GGA-AGC | |||
| AggRkas2 | 353–334 | ACA-GAA-TCG-TCA-GCA-TCA-GC | aggR | 254 | 26 |
| PetS | 557–576 | TCA-TTT-CCA-GCA-CTT-CCT-GT | |||
| PetAS | 979–998 | CTC-CGA-CAG-TAT-TTG-CTC-GT | pet | 442 | This study |