FIG. 2.
Growth of B. anthracis Sterne 7702 and srtA mutant strains in J774A.1 cells. J774A.1 cells (7.5 × 105 cells/well) were infected at an MOI of 1 with either B. anthracis Sterne 7702 (•), the srtA mutant strain B. anthracis Sterne 7702::pHYZ2(pUTE29) (▪), or the srtA mutant strain complemented with the srtA gene, B. anthracis Sterne 7702::pHYZ2(pEZ31) (▴). At each time point, the number of intracellular (A) and extracellular (B) bacteria were determined as described in the text. Data points and error bars represent mean CFU of the triplicate samples ± standard deviation. The experiment was performed three times, and the results shown are representative of those obtained in each experiment.