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. 2005 Aug;73(8):4596–4606. doi: 10.1128/IAI.73.8.4596-4606.2005

FIG. 5.

FIG. 5.

Effect of heparin on known biofilm formation mutants. A. Mutants (gene names noted) were assessed for biofilm formation in the microtiter dish assay (8-h biofilms) with heparin at 1,000 U/ml (shaded bars) or with saline (white bars). B. Relative PIA levels were determined in response to heparin at 1,000 U/ml. Tenfold serial dilutions of protease-treated whole-cell lysates were immunoblotted with anti-PIA antibodies. C. Isogenic pairs of wild-type and ica mutants in the MZ100 and Sa113 backgrounds were assessed for biofilm formation in the microtiter dish assay (9-h biofilms). One asterisk signifies statistical significance (P < 0.05) between a wild-type strain and its isogenic mutant strain without heparin; two asterisks signify statistical significance (P < 0.05) between strains with and without heparin.