FIG. 5.
Effect of caspase inhibitors on ΔΨm after incubation of THP-1 cells with Stx1. THP-1 cells were incubated with the caspase-8 inhibitor (C8I) or the general caspase inhibitor (ZVAD-fmk) for 1 h before treatment with Stx1 (400 ng/ml). Caspase inhibitors were used at 40 μM. After 8 h of incubation with Stx1, cells were stained with JC-1 reagent and analyzed by flow cytometry. Control cells were not treated with Stx1. The percentages of cells staining for red and green fluorescence and green fluorescence only are shown in the scatter plots. The data shown are representative of at least two independent experiments.