FIG. 6.

(A) Quantification of DEC8B and Texas outbreak strains adherent to cultured HeLa cells after 3 h of incubation in the absence or presence of cadaverine (300 and 500 μM). The error bars indicate standard deviations. (B) Effect of different concentrations of cadaverine on expression of intimin in the DEC8B (lanes 1 through 4), DEC8E (lanes 5 through 8), and Texas outbreak (lanes 9 though 12) strains. Whole-cell lysates of bacterial samples in the absence (lanes 1, 5, and 9) or presence of 300 μM (lanes 2, 6, and 10), 500 μM (lanes 3, 7, and 11), or 900 μM (lanes 4, 8, and 12) of cadaverine were fractionated by SDS-10% PAGE and anti-intimin serum was used for the Western blot experiment. M, molecular size standards in kilodaltons. The intimin protein is identified with a black arrow.