FIG. 1.

Protection against visceral leishmaniasis in LACK DNA-LACK vaccinia virus-vaccinated mice. Shown are the results of parasite burden analyses of BALB/c mice vaccinated with a prime-boost regimen (DNA genes and recombinant vaccinia virus [VV]) using the LACK antigen and then infected intradermally with L. infantum promastigotes. Parasite burdens were determined using limiting dilution analyses and represent the averaged values for at least four mice/group. (A) Spleen; (B) liver; (C) lymph node. Statistical analyses were performed using Student's t test comparing vaccine groups to a vector control group (pCI-neo-WRLuc). ***, P < 0.001; **, P < 0.01; *, P < 0.05. PBS, phosphate-buffered saline.