FIG. 2.

Antigen-specific IFN-γ, TNF-α, and IL-10 responses in prime-boost vaccinated mice prior to infection. Shown are the IFN-γ, TNF-α, and IL-10 responses found for the vaccinated and control groups of mice (as indicated) prior to infection. The results of (A) ELISAs of IFN-γ from culture supernatants of splenic cells in response to recombinant LACK antigen, (B) corresponding enzyme-linked immunospot analyses of IFN-γ, and ELISAs of (C) TNF-α/LT and (D) IL-10 are shown. PBS, phosphate-buffered saline.